Journal:

Article Title: Importance of the Endocarditis and Biofilm-associated Pilus ( ebp ) Locus in the Pathogenesis of Enterococcus faecalis Ascending Urinary Tract Infection

doi: 10.1086/517524

Figure Lengend Snippet: Mixed infection (competition assay) by w.t. E. faecalis OG1RF and nonpiliated TX5475 in the kidneys and urinary bladder of mice (n=16). Data are expressed as log10CFU of w.t. E. faecalis OG1RF or TX5475. The log10CFU/gm from both kidneys were combined and averaged. Solid diamonds and triangles represent w.t. E. faecalis OG1RF and TX5475, respectively, from kidney homogenates and empty diamonds and triangles represent w.t. E. faecalis OG1RF and TX5475, respectively, from urinary bladder homogenates. Horizontal bars represent geometric mean titer. Mean fold difference in CFU are given. Log10 CFU were compared for statistical significance using a paired t test.

Article Snippet: Bacterial strains and growth conditions Bacteria used in the study include wild type OG1RF (resistant to rifampicin 100 μg/ml, fusidic acid 25 μg/ml) (ATCC 47077), a well-known plasmid-free isolate used in many laboratories [ 13 ]; OG1RF is a medium biofilm producer [ 7 ] and has been shown to be pathogenic in various animal models [ 12 , 14 - 17 ].

Techniques: Infection, Competitive Binding Assay

Journal:

Article Title: Importance of the Endocarditis and Biofilm-associated Pilus ( ebp ) Locus in the Pathogenesis of Enterococcus faecalis Ascending Urinary Tract Infection

doi: 10.1086/517524

Figure Lengend Snippet: Tropism for the kidneys by E. faecalis strains OG1RF and its isogenic nonpiliated Δ ebpA (TX5475) mutant in a murine model of ascending urinary tract infection

Article Snippet: Bacterial strains and growth conditions Bacteria used in the study include wild type OG1RF (resistant to rifampicin 100 μg/ml, fusidic acid 25 μg/ml) (ATCC 47077), a well-known plasmid-free isolate used in many laboratories [ 13 ]; OG1RF is a medium biofilm producer [ 7 ] and has been shown to be pathogenic in various animal models [ 12 , 14 - 17 ].

Techniques: Mutagenesis, Infection

Journal:

Article Title: Importance of the Endocarditis and Biofilm-associated Pilus ( ebp ) Locus in the Pathogenesis of Enterococcus faecalis Ascending Urinary Tract Infection

doi: 10.1086/517524

Figure Lengend Snippet: Monoinfection (106, 105, 104, and 103 inocula) using w.t. E. faecalis OG1RF or nonpiliated TX5475 in the kidneys of mice (n=10). Data are expressed as log10CFU/gm of w.t. E. faecalis OG1RF or TX5475 recovered from kidney homogenates 48 hrs after transurethral challenge. The log10CFU from both kidneys were combined and averaged. Solid and empty triangles represent w.t. E. faecalis OG1RF and TX5475, respectively. Horizontal bars represent geometric mean titer. Mean difference in CFU is given as log10 ± standard deviation (SD) for the respective inocula. TX5475 CFUs (recovered from kidneys) were significantly reduced versus OG1RF with all four inocula using unpaired t test.

Article Snippet: Bacterial strains and growth conditions Bacteria used in the study include wild type OG1RF (resistant to rifampicin 100 μg/ml, fusidic acid 25 μg/ml) (ATCC 47077), a well-known plasmid-free isolate used in many laboratories [ 13 ]; OG1RF is a medium biofilm producer [ 7 ] and has been shown to be pathogenic in various animal models [ 12 , 14 - 17 ].

Techniques: Standard Deviation

Journal: Cell chemical biology

Article Title: Chemical induction of aminoglycoside uptake overcomes antibiotic tolerance and resistance in Staphylococcus aureus

doi: 10.1016/j.chembiol.2019.07.009

Figure Lengend Snippet: Tobramycin/rhamnolipid MIC values for other gram positive and negative bacterial species (µg/mL)

Article Snippet: Enterococcus faecalis wild-type OG1 , ATCC , ATCC 47077.

Techniques:

Journal: Cell chemical biology

Article Title: Chemical induction of aminoglycoside uptake overcomes antibiotic tolerance and resistance in Staphylococcus aureus

doi: 10.1016/j.chembiol.2019.07.009

Figure Lengend Snippet: Key Resources Table

Article Snippet: Enterococcus faecalis wild-type OG1 , ATCC , ATCC 47077.

Techniques: Plasmid Preparation, Derivative Assay, Isolation, Cloning, Recombinant, Modification, Membrane, Viability Assay, Software, Flow Cytometry